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10X Genomics cellranger 10x genomics cell ranger 3.0.0
Cellranger 10x Genomics Cell Ranger 3.0.0, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Recombinant:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Virus:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Control:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Labeling:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Amplification:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Binding Assay:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Clone Assay:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Transfection:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Gel Extraction:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Nested PCR:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Sequencing:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Expressing:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Plasmid Preparation:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Software:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Transmission Assay:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Microscopy:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Purification:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Formalin-fixed Paraffin-Embedded:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Staining:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Conjugation Assay:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

RNAscope:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Multiplex Assay:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1

Generated:

Article Title: Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , N/A.

Article Title: Astrocyte transcriptomic changes along the spatiotemporal progression of Alzheimer’s disease
Article Snippet: Raw reads were processed using Cell Ranger 3.0.0 (10x Genomics) with default settings, pre-mRNA package and aligned to the human GRCh38 genome.

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle
Article Snippet: Cell Ranger 3.0.0 , 10X Genomics , support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model.
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/) was used to process the raw sequencing data.

Article Title: Single-Cell RNA Profiling of Ocular Adnexal Sebaceous Carcinoma Reveals a Complex Tumor Microenvironment and Identifies New Biomarkers.
Article Snippet: • PURPOSE: Ocular adnexal sebaceous carcinoma (OaSC) is an aggressive malignancy that often necessitates orbital exenteration.. Its tumor composition and transcriptional profile remain largely unknown, which poses a significant barrier to medical advances.. Here, we report the first in-depth transcriptomic analysis of OaSC at the single-cell resolution and discern mechanisms underlying cancer progression for the discovery of potential globe-sparing immunotherapies, targeted therapies, and biomarkers to guide clinical management.

Article Title: Single Cell RNA Sequencing Analysis of Mouse Retina Identifies a Subpopulation of Muller Glia Involved in Retinal Recovery From Injury in the FCD-LIRD Model
Article Snippet: Cell Ranger 3.0.0 (10X Genomics, https://www.10xgenomics.com/ ) was used to process the raw sequencing data.

Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Purified antibodies Various See Table S7 for details Biological samples FFPE tissue samples National Human Genetic Resources Sharing Service Platform See Table S2A for details Fresh tissue samples Sun Yat-sen University Cancer Center See Table S6 for details Critical commercial assays Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 eBioscience Foxp3 / Transcription Factor Staining Buffer Set Invitrogen Cat#00-5523-00 PanoPANEL Kits Panovue Cat#10234100050 PhenoCycler-Fusion Conjugation Kit Akoya Biosciences Cat#7000009 Deposited data Data files for human scRNA-seq dataset This study GEO: GSE233236 Raw data for human scRNA-seq dataset This study GSA for human: HRA000321 Public human cancer scRNA-seq datasets Various public studies See Table S1A for details SMART-seq2 scRNA-seq datasets Various public studies See Table S5A for details scRNA-seq datasets from non-cancer conditions Various public studies See Table S5B for details Human BCRA scATAC-seq dataset Zhang et al.51 GEO: GSE169246 Human melanoma anti-PD-1 treatment dataset Liu et al.115 dbGAP: phs000452.v3.p1 Human melanoma anti-CTLA-4 treatment dataset Van Allen et al.116 dbGaP: phs000452.v2.p1 Human melanoma adoptive cell transfer treatment dataset Lauss et al.117 GEO: GSE100797 Human lung cancer anti-PD-1 treatment dataset Cho et al.118 GEO: GSE126044 Human melanoma anti-PD-1 treatment dataset Gide et al.119 ENA: PRJEB23709 Human melanoma anti-CTLA-4 treatment dataset Auslander et al.120 GEO: GSE115821 Human lung cancer anti-PD-1 treatment dataset Prat et al.121 GEO: GSE93157 Human pan-cancer B cell scRNA-seq dataset Ma et al.109 http://pancancer.cn/B/ Software and algorithms ImageScope v12.4.3.5008 Leica Biosystems https://www.leicabiosystems.com/digital- pathology/manage/aperio-imagescope/ OlyVIA 3.3 OLYMPUS https://olyvia.software.informer.com/ HALO 3.5 Indica Labs https://www.indicalab.com/halo HighPlex FL 4.2.3 Indica Labs https://indicalab.com/products/high-plex- fl/ Cell Ranger 3.0.0 10x Genomics https://10xgenomics.com/ R 4.1.2 R Core Team122 https://www.r-project.org/ Python 3.8.0 Python Software Foundation123 https://www.python.org/ (Continued on next page) Cell 187, 1–22.e1–e9, August 22, 2024 e1



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